Chemosensitivity: Volume I: In Vitro Assays - Methods in Molecular Medicine 2005 edition

Marc Notes: v. 1. In vitro assays -- v. 2 In vivo models, imaging, and molecular regulators; Bibl. ref. & index. Table of Contents: Part I. Overview An Overview of Chemosensitivity Testing Rosalyn D. Blumenthal Part II. In Vitro Measures of Chemosensitivity Clonogenic Cell Survival Assay Anupama Munshi, Marvette Hobbs, and Raymond E. Meyn High-Sensitivity Cytotoxicity Assays for Nonadherent Cells M. Jules Mattes Sulforhodamine B Assay and Chemosensitivity Wieland Voight Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity Gertjan J. L. Kaspers Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity Hisayuki Kobayashi The MTT Assay to Evaluate Chemosensitivity Jack D. Burton Histoculture Drug Response Assay to Monitor Chemoresponse Shinji Ohie, Yasuhiro Udagawa, Daisuke Aoki, and Shiro Nozawa In Vitro Testing of Chemosensitivity in Physiological Hypoxia Rita Grigoryan, Nino Keshelava, Clarke Anderson, and C. Patrick Reynolds Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements Christian M. Kurbacher and Ian A. Cree High-Throughput Technology: Green Fluorescent Protein to Monitor Cell Death Marylene Fortin, Ann-Muriel Steff, and Patrice Hugo DIMSCAN: A Microcomputer Fluorescence-Based Cytotoxicity Assay for Preclinical Testing of Combination Chemotherapy Nino Keshelava, Tomas Frgala, JirD i Krejsa, Ondrej Kalous, and C. Patrick Reynolds The ChemoFx(r) Assay: An Ex Vivo Cell Culture Assay for Predicting Anticancer Drug Responses Robert L. Ochs, Dennis Burholt, and Paul Kornblith Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models C. Patrick Reynolds and Barry J. Maurer Image Analysis Using the Fluochromasia Assay to Quantify Tumor Drug Sensitivity John F. Gibbs, Youcef M. Rustum, and Harry K. Slocum ImmunohistochemicalDetection of Ornithine Decarboxylase as a Measure of Chemosensitivity Uriel Bachrach Immunohistochemistry of p53, Bcl-2 and Ki-67 as Predictors of Chemosensitivity Mitsuyoshi Itaya, Jiro Yoshimoto, Kuniaki Kojima, and Seiji Kawasaki Index"Jacket Description/Back: Chemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe state-of-the-art laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and nonadherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients. Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more, or less, sensitive to a particular agent."