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Membrane Proteins of an Antarctic Bacterium Pseudomonas Syringae: Proteomics, Membrane Proteome, Antarctic Bacterium, Pseudomonas Syringae Lz4w, Maldi Tof / Tof Lc-esi Ms/ms, Electrophoresis, Detergents
Medicharla V Jagannadham
Membrane Proteins of an Antarctic Bacterium Pseudomonas Syringae: Proteomics, Membrane Proteome, Antarctic Bacterium, Pseudomonas Syringae Lz4w, Maldi Tof / Tof Lc-esi Ms/ms, Electrophoresis, Detergents
Medicharla V Jagannadham
Identification of membrane proteins is the main focus of the present study. Preparation, separation and identification of membrane proteins remain a highly challenging and interesting task. In proteomics, proteins are usually identified using mass spectrometry and identify proteins by comparing with existing protein or genome databases. This book deals with the twin objectives of efficient membrane protein preparation in general, as well as identification of proteins from species of unknown genome sequences, using an Antarctic bacterium Pseudomonas syringae, as a model system. Using a combination of de novo sequencing and chemical modification of tryptic peptides followed by mass spectrometry, the peptide sequence was determined/validated. It is hoped that the procedures developed and applied in this study would be useful to a large number of researchers working in diverse areas of Microbiology, Chemistry, Biochemistry, Biotechnology, Bioinformatics, Proteomics and Mass spectrometry.
Media | Books Paperback Book (Book with soft cover and glued back) |
Released | April 27, 2011 |
ISBN13 | 9783639335828 |
Publishers | VDM Verlag Dr. Müller |
Pages | 188 |
Dimensions | 150 × 11 × 226 mm · 281 g |
Language | English |
See all of Medicharla V Jagannadham ( e.g. Paperback Book )