Interferon-gamma: a Marker of Tuberculosis Mediated Immune Response: University of Health Sciences, Lahore, Pakistan

Background: Interferon gamma (IFN-?), a cytokine produced by a variety of cells is involved in the immune response against M.tuberculosis. It activates the production of other cytokines & molecules that kill MTB. IFN-? also has diagnostic role in identification of active and latent TB. Objective: To determine the level of IFN-? in the blood of TB patients. Methods: 91 subjects were selected, including 54 active TB patients and 37 healthy controls. IFN-? conc. was determined in their blood by ELISA technique. Results: In TB patients, Mean+SD of IFN-? was 48.69+28.78 while it was 12.99+5.70 pg/ml in control group (p <0.001). Significant differences in the level of IFN-? were observed among confirmed TB patients, clinically diagnosed TB patients and the control group (Mean+SD 59.68+28.78, 36.85+24.76 and 12.99+5.70 pg/ml, respectively). Furthermore, a significant negative correlation was observed b/w conc. of IFN-? in TB patients and the duration of anti-tuberculosis therapy. Conclusion: IFN-? level was high in both clinically diagnosed and confirmed TB patients as compared to a control group. Measurement of IFN-? is helpful to diagnose active TB, but further research is required.